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monoclonal antibody da2b11  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank monoclonal antibody da2b11
Response of endogenous ADIP to wound healing. (A-C) Immunostaining of stage 12 embryos reveals ADIP puncta in ectoderm cells (A). Cell borders are marked by Wtip (A′); merged image (A″) shows partial colocalization of ADIP and Wtip puncta in the apical domain (white arrows). (B) Segmented cell outlines show random orientation of ADIP polarity vectors (arrows). (C) Rose plot confirms random distribution. (D-F) Stage 12 embryos were wounded on the ventral animal side and allowed to heal for 30 min in 0.7× MMR. ADIP becomes polarized in the cells proximal to the wound margin (D). Wtip labels cell borders (D′); merged image (D″). Arrows in D indicate ADIP puncta and polarized Wtip at cell junction facing the wound. Scale bar in D″: 20 μm; the same scale applies to panels A-A′′, B, E, G-G′, H-H′. Cell segmentation (E) and rose plot (F) show ADIP polarity vectors (arrows in E) oriented toward the wound site (0°). Rose plot represents pooled polarity from two embryos. Chi-square test indicates a non-random distribution, P <0.05. (G-I) Anti-Wtip staining of stage 10.5 embryos co-injected with 20 ng of control MO (G-G′) or Wtip MO (H-H′) and 50 pg H2B-GFP RNA as lineage tracer. MO-injected cells are marked by rabbit polyclonal antibody for GFP; merged images in G′,H′. (I) Immunoblotting of endogenous Wtip in stage 13 embryo lysates injected with control MO or Wtip MO. Blots were probed with mouse anti-Wtip <t>(DA2B11)</t> antibody (upper panel) and anti-β-catenin antibody as a loading control (lower panel). Data shown are representative of three independent experiments, each performed on 10-15 embryos.
Monoclonal Antibody Da2b11, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wilms+tumor/anti-Wilms+tumor+protein+1-interacting+protein+homolog/pmc12919953-190-5-12
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monoclonal antibody da2b11 - by Bioz Stars, 2026-08
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wilms tumor 1 antibody  (NSJ Bioreagents)
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Response of endogenous ADIP to wound healing. (A-C) Immunostaining of stage 12 embryos reveals ADIP puncta in ectoderm cells (A). Cell borders are marked by Wtip (A′); merged image (A″) shows partial colocalization of ADIP and Wtip puncta in the apical domain (white arrows). (B) Segmented cell outlines show random orientation of ADIP polarity vectors (arrows). (C) Rose plot confirms random distribution. (D-F) Stage 12 embryos were wounded on the ventral animal side and allowed to heal for 30 min in 0.7× MMR. ADIP becomes polarized in the cells proximal to the wound margin (D). Wtip labels cell borders (D′); merged image (D″). Arrows in D indicate ADIP puncta and polarized Wtip at cell junction facing the wound. Scale bar in D″: 20 μm; the same scale applies to panels A-A′′, B, E, G-G′, H-H′. Cell segmentation (E) and rose plot (F) show ADIP polarity vectors (arrows in E) oriented toward the wound site (0°). Rose plot represents pooled polarity from two embryos. Chi-square test indicates a non-random distribution, P <0.05. (G-I) Anti-Wtip staining of stage 10.5 embryos co-injected with 20 ng of control MO (G-G′) or Wtip MO (H-H′) and 50 pg H2B-GFP RNA as lineage tracer. MO-injected cells are marked by rabbit polyclonal antibody for GFP; merged images in G′,H′. (I) Immunoblotting of endogenous Wtip in stage 13 embryo lysates injected with control MO or Wtip MO. Blots were probed with mouse anti-Wtip <t>(DA2B11)</t> antibody (upper panel) and anti-β-catenin antibody as a loading control (lower panel). Data shown are representative of three independent experiments, each performed on 10-15 embryos.
Wilms Tumor 1 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wilms+tumor/Wilms+Tumor+1+Antibody/custom%40v2930%4042488332
Average 99 stars, based on 1 article reviews
wilms tumor 1 antibody - by Bioz Stars, 2026-08
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rabbit anti wilm s tumor  (Spring Bioscience)
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Response of endogenous ADIP to wound healing. (A-C) Immunostaining of stage 12 embryos reveals ADIP puncta in ectoderm cells (A). Cell borders are marked by Wtip (A′); merged image (A″) shows partial colocalization of ADIP and Wtip puncta in the apical domain (white arrows). (B) Segmented cell outlines show random orientation of ADIP polarity vectors (arrows). (C) Rose plot confirms random distribution. (D-F) Stage 12 embryos were wounded on the ventral animal side and allowed to heal for 30 min in 0.7× MMR. ADIP becomes polarized in the cells proximal to the wound margin (D). Wtip labels cell borders (D′); merged image (D″). Arrows in D indicate ADIP puncta and polarized Wtip at cell junction facing the wound. Scale bar in D″: 20 μm; the same scale applies to panels A-A′′, B, E, G-G′, H-H′. Cell segmentation (E) and rose plot (F) show ADIP polarity vectors (arrows in E) oriented toward the wound site (0°). Rose plot represents pooled polarity from two embryos. Chi-square test indicates a non-random distribution, P <0.05. (G-I) Anti-Wtip staining of stage 10.5 embryos co-injected with 20 ng of control MO (G-G′) or Wtip MO (H-H′) and 50 pg H2B-GFP RNA as lineage tracer. MO-injected cells are marked by rabbit polyclonal antibody for GFP; merged images in G′,H′. (I) Immunoblotting of endogenous Wtip in stage 13 embryo lysates injected with control MO or Wtip MO. Blots were probed with mouse anti-Wtip <t>(DA2B11)</t> antibody (upper panel) and anti-β-catenin antibody as a loading control (lower panel). Data shown are representative of three independent experiments, each performed on 10-15 embryos.
Rabbit Anti Wilm S Tumor, supplied by Spring Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wilms+tumor/anti+rabbit+s+tumor+wilm/us12653843-478-47-52
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rabbit anti wilm s tumor - by Bioz Stars, 2026-08
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tumor protein 1 wt1  (Boster Bio)
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Boster Bio tumor protein 1 wt1
Response of endogenous ADIP to wound healing. (A-C) Immunostaining of stage 12 embryos reveals ADIP puncta in ectoderm cells (A). Cell borders are marked by Wtip (A′); merged image (A″) shows partial colocalization of ADIP and Wtip puncta in the apical domain (white arrows). (B) Segmented cell outlines show random orientation of ADIP polarity vectors (arrows). (C) Rose plot confirms random distribution. (D-F) Stage 12 embryos were wounded on the ventral animal side and allowed to heal for 30 min in 0.7× MMR. ADIP becomes polarized in the cells proximal to the wound margin (D). Wtip labels cell borders (D′); merged image (D″). Arrows in D indicate ADIP puncta and polarized Wtip at cell junction facing the wound. Scale bar in D″: 20 μm; the same scale applies to panels A-A′′, B, E, G-G′, H-H′. Cell segmentation (E) and rose plot (F) show ADIP polarity vectors (arrows in E) oriented toward the wound site (0°). Rose plot represents pooled polarity from two embryos. Chi-square test indicates a non-random distribution, P <0.05. (G-I) Anti-Wtip staining of stage 10.5 embryos co-injected with 20 ng of control MO (G-G′) or Wtip MO (H-H′) and 50 pg H2B-GFP RNA as lineage tracer. MO-injected cells are marked by rabbit polyclonal antibody for GFP; merged images in G′,H′. (I) Immunoblotting of endogenous Wtip in stage 13 embryo lysates injected with control MO or Wtip MO. Blots were probed with mouse anti-Wtip <t>(DA2B11)</t> antibody (upper panel) and anti-β-catenin antibody as a loading control (lower panel). Data shown are representative of three independent experiments, each performed on 10-15 embryos.
Tumor Protein 1 Wt1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wilms+tumor/Anti-Wilms+tumor+protein+WT1+Antibody/pm41828705-139-24-29
Average 94 stars, based on 1 article reviews
tumor protein 1 wt1 - by Bioz Stars, 2026-08
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Image Search Results


Response of endogenous ADIP to wound healing. (A-C) Immunostaining of stage 12 embryos reveals ADIP puncta in ectoderm cells (A). Cell borders are marked by Wtip (A′); merged image (A″) shows partial colocalization of ADIP and Wtip puncta in the apical domain (white arrows). (B) Segmented cell outlines show random orientation of ADIP polarity vectors (arrows). (C) Rose plot confirms random distribution. (D-F) Stage 12 embryos were wounded on the ventral animal side and allowed to heal for 30 min in 0.7× MMR. ADIP becomes polarized in the cells proximal to the wound margin (D). Wtip labels cell borders (D′); merged image (D″). Arrows in D indicate ADIP puncta and polarized Wtip at cell junction facing the wound. Scale bar in D″: 20 μm; the same scale applies to panels A-A′′, B, E, G-G′, H-H′. Cell segmentation (E) and rose plot (F) show ADIP polarity vectors (arrows in E) oriented toward the wound site (0°). Rose plot represents pooled polarity from two embryos. Chi-square test indicates a non-random distribution, P <0.05. (G-I) Anti-Wtip staining of stage 10.5 embryos co-injected with 20 ng of control MO (G-G′) or Wtip MO (H-H′) and 50 pg H2B-GFP RNA as lineage tracer. MO-injected cells are marked by rabbit polyclonal antibody for GFP; merged images in G′,H′. (I) Immunoblotting of endogenous Wtip in stage 13 embryo lysates injected with control MO or Wtip MO. Blots were probed with mouse anti-Wtip (DA2B11) antibody (upper panel) and anti-β-catenin antibody as a loading control (lower panel). Data shown are representative of three independent experiments, each performed on 10-15 embryos.

Journal: Biology Open

Article Title: Planar polarization of endogenous ADIP during Xenopus neurulation

doi: 10.1242/bio.062452

Figure Lengend Snippet: Response of endogenous ADIP to wound healing. (A-C) Immunostaining of stage 12 embryos reveals ADIP puncta in ectoderm cells (A). Cell borders are marked by Wtip (A′); merged image (A″) shows partial colocalization of ADIP and Wtip puncta in the apical domain (white arrows). (B) Segmented cell outlines show random orientation of ADIP polarity vectors (arrows). (C) Rose plot confirms random distribution. (D-F) Stage 12 embryos were wounded on the ventral animal side and allowed to heal for 30 min in 0.7× MMR. ADIP becomes polarized in the cells proximal to the wound margin (D). Wtip labels cell borders (D′); merged image (D″). Arrows in D indicate ADIP puncta and polarized Wtip at cell junction facing the wound. Scale bar in D″: 20 μm; the same scale applies to panels A-A′′, B, E, G-G′, H-H′. Cell segmentation (E) and rose plot (F) show ADIP polarity vectors (arrows in E) oriented toward the wound site (0°). Rose plot represents pooled polarity from two embryos. Chi-square test indicates a non-random distribution, P <0.05. (G-I) Anti-Wtip staining of stage 10.5 embryos co-injected with 20 ng of control MO (G-G′) or Wtip MO (H-H′) and 50 pg H2B-GFP RNA as lineage tracer. MO-injected cells are marked by rabbit polyclonal antibody for GFP; merged images in G′,H′. (I) Immunoblotting of endogenous Wtip in stage 13 embryo lysates injected with control MO or Wtip MO. Blots were probed with mouse anti-Wtip (DA2B11) antibody (upper panel) and anti-β-catenin antibody as a loading control (lower panel). Data shown are representative of three independent experiments, each performed on 10-15 embryos.

Article Snippet: Data and resource availability The monoclonal antibody DA2B11 has been deposited in Developmental studies hybridoma bank (DSHB).

Techniques: Immunostaining, Staining, Injection, Control, Western Blot